Epstein-Barr virus BZLF1 trans activator induces the promoter of a cellular cognate gene, c-fos.

Flemington E; Speck SH

Division of Tumor Virology, Dana-Farber Cancer Institute, Boston, Massachusetts.

J Virol 64: 4549-52 (1990)

Abstract
The Epstein-Barr virus BZLF1 gene product ZEBRA is a DNA-binding protein that is partially homologous to c-Fos, binds specifically to AP-1 sites, and can induce the lytic cycle in latently infected B lymphocytes. Induction of the viral lytic cycle can also be achieved by treatment with the phorbol ester 12-O-tetrade-canoylphorbol-13-acetate, a reagent which activates gene expression in part through AP-1 (Jun/Fos). In this article the interrelationship between ZEBRA and AP-1 is extended by the demonstration that ZEBRA can induce c-Fos expression through AP-1 and "AP-1-like" sites present in the c-fos promoter. Induction of c-Fos may be necessary for the expression of other viral lytic genes and perhaps cellular genes whose products are required for viral replication.

Mesh Headings

Base Sequence
Cell Line
Comparative Study
DNA-Binding Proteins*
Enhancer Elements (Genetics)
Epstein-Barr Virus*
Hela Cells
Human
Molecular Sequence Data
Mutation
Promoter Regions (Genetics)*
Protein-Tyrosine Kinase
Proto-Oncogene Proteins, Cellular*
Proto-Oncogenes*
Sequence Homology, Nucleic Acid
Support, U.S. Gov't, P.H.S.
Trans-Activators*

Unique Identifier: 90347854

Chemical Identifiers (Names)

EC 2.7.1.112 (Protein-Tyrosine Kinase)
(proto-oncogene protein fos)
(BZLF1 protein)