The Epstein-Barr virus bZIP transcription factor Zta causes G0/G1 cell cycle arrest through induction of cyclin-dependent kinase inhibitors.

Cayrol C; Flemington EK

Division of Tumor Virology, Division of Neoplastic Disease Mechanisms, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA.

EMBO J 15: 2748-59 (1996)

Abstract
While oncoproteins encoded by small DNA tumor viruses and Epstein-Barr virus (EBV) latent antigens facilitate G1/S progression, the EBV lytic switch transactivator Zta was found to inhibit growth by causing cell cycle arrest in G0/G1 in several epithelial tumor cell lines. Expression of Zta results in induction of the tumor suppressor protein, p53, and the cyclin-dependent kinase inhibitors, p21 and p27, as well as accumulation of hypophosphorylated pRb. Up-regulation of p53 and p27 occurs by post-transcriptional mechanisms while expression of p21 is induced at the RNA level in a p53-dependent manner. Inactivation of pRb by transient overexpression of the human papillomavirus E7 oncoprotein indicates that pRb or pRb-related proteins are key mediators of the growth-inhibitory function of Zta. These findings suggest that EBV plays an active role in redirecting epithelial cell physiology to facilitate the viral replicative program through a Zta-mediated growth arrest function.

Mesh Headings

Cell Cycle*
Cyclin-Dependent Kinases*
Cyclins*
DNA
DNA-Binding Proteins*
Growth Inhibitors*
Hela Cells
Herpesvirus 4, Human*
Human
Leucine Zippers
Microtubule-Associated Proteins*
Nasopharyngeal Neoplasms
Protein p53
Retinoblastoma Protein
Support, Non-U.S. Gov't
Support, U.S. Gov't, P.H.S.
Trans-Activators*
Tumor Cells, Cultured
Viral Proteins
Virus Replication

Unique Identifier: 96256292

Chemical Identifiers (Names)

(BZLF1 protein)
(Cip1 protein)
(Cyclin-Dependent Kinases)
(Cyclins)
(DNA-Binding Proteins)
(Growth Inhibitors)
(Microtubule-Associated Proteins)
(Protein p53)
(Retinoblastoma Protein)
(Trans-Activators)
(Viral Proteins)
147604-94-2 (KIP1 protein)
9007-49-2 (DNA)